Collagen scaffold facilitates the three-dimensional culture of myocardial cells from adult rats in vitro

doi:10.3969/j.issn.2095-4344.2013.16.016

Abstract

Abstract:

BACKGROUND: Three-dimensional contractible myocardial cell block of neonatal rats in the Atelocollagen scaffold has been cultured in the previous study.
OBJECTIVE: To perform three-dimensional culture of myocardial cells from adult rats on the Atelocollagen scaffold.
METHODS: After being cultured and purified, myocardial cells from adult Sprague-Dawley rats were implanted onto the Atelocollagen scaffold for three-dimensional cultures. The growth of myocardial cells on the Atelocollagen scaffold was dynamically observed by inverted microscopy. Myocardial cell blocks were separately tested by hematoxylin-eosin staining, immunohistochemistry and immunofluorecence of troponin after frozen section.
RESULTS AND CONCLUSION: After being implanted, myocardial cells from adult Sprague-Dawley rats began to grow on the Atelocollagen scaffold in 12 hours, showed mutual integration with the scaffold and the integration became more closely 24 hours later. However, the myocardial cells did not have autorhythmiciting beats at all, and among the cells there were some cell debris. After replaced with the same fresh medium 48 hours later, the cell debris was washed away and most of the rest cells grew on the edge of the Atelocollagen scaffold. After 48 hours, the cells formed a complex with the scaffold tested by hematoxylin-eosin staining, and the cells which grew well and were coalesced with the scaffold meshes were mainly myocardial cells, identified by immunohistochemistry and immunofluorecence.

Key words: biomaterials, extracellular matrix materials, collagen scaffold, myocardial cells, three-dimensional culture, immunohistochemistry, immunofluorecence, adult rats, provincial grants-supported paper

CLC Number:

R318

Wang Hui-ling, Li Qiong, Guo Zhi-kun, Sun Yong-kun, Kuboki Y. Collagen scaffold facilitates the three-dimensional culture of myocardial cells from adult rats in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2961-2967.

Figures/Tables 7

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